Haemoglobin Binding with Haptoglobin UNEQUIVOCAL DEMONSTRATION THAT THE f-CHAINS OF HUMAN HAEMOGLOBIN
نویسندگان
چکیده
Haptoglobin binding to haemoglobin and its isolated a and #-chains was studied by use of a highly sensitive solid-phase radiometric assay. As expected, adsorbents of haemoglobin bound '25I-labelled haptoglobin more efficiently than did adsorbents of the (achain. However, unexpectedly, adsorbents of the f-chain were found to be essentially identical with those of the a-chain in their ability to bind haptoglobin. These results demonstrate, unequivocally, the ability of fl-chains to bind to haptoglobin, and indicate that this assay is particularly convenient and useful for studying haptoglobin interactions with haemoglobin and its aand fl-chains.
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